Nanoscale Imaging And Characterisation Of Amyloid- (springer Theses)
by Claire Louisa Tinker-Mill /
2016 / English / PDF
6.2 MB Download
Authors: Tinker-Mill, Claire Louisa
Nominated as an outstanding Ph.D. thesis by the Lancaster University, UK
Presents a detailed study of amyloid-beta using multiple methods of atomic force microscopy
Author received the 2012 and 2014 Juno Award for Research Excellence from Lancaster University
This thesis presents a method for reliably and robustly producing samples of amyloid- (A) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptides nanomechanical, elastic, thermal or spectroscopical properties.
Amyloid- (A) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of A with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.
Number of Illustrations and Tables
45 b/w illustrations, 14 illustrations in colour
Topics
Spectroscopy and Microscopy
Soft and Granular Matter, Complex Fluids and Microfluidics
Molecular Medicine
Neurology
Nanotechnology
